1Alaa Hassan Khaliel, 2Ahmed Abdul- Hassan Abbas, 3Anmar Oday Hatem
1Ministry of Health, Baghdad, Iraq; 2College of Medicine, Al-Nahrain University, Baghdad, Iraq; 3Baghdad Teaching Hospital, Baghdad, Iraq
Background:
To assess the genotypes of (HLA)-DRB1 * 15:01 as genetic risk factor for MS development in samples of Iraqi MS patients.
Material(s) and Method(s):
This Case control study involved; fifty MS patients for HLA-DRB1 15:01 investigation; their age were ranged from 14 to 69 years. They attended to seek treatment in the MS out patient’s clinic at Medical City- Baghdad Teaching Hospital in the period, which extended from December 2018 to March 2020. The diagnosis of each case was established according to MC Donald criteria done by a neurologist and confirmed by MRI and certain cases by oligoclonalband test of the CSF. Patients were subjected to a questionnaire about name, age, sex, smoking, family history, the control group involved 50 apparently healthy person. Patients were. The institutional review board (IRB) in the College of Medicine/Al-Nahrain University approved this study, and all samples were obtained with informed consent in accordance with the Ministry of Health declaration. HLA-DRB1 15:01 genotype also was conducted by Sanger sequencing technique.
Result(s):
The Polymerase chain reaction (PCR) products HLA-DRB1 Genes were subjected for Sanger sequencing technique. In addition, the resultant sequences were compared with reference sequences in national center for biotechnology information NCBI. All the genotypes of HLA-DRB1 were analyzed for linkage disequilibrium. There was a very high linkage disequilibrium in rs2213585, rs2213586 and rs3135388 in both patients and control. In concern to the rs3135388, which tags for HLA-DRB1*15:01 the heterozygous genotype (GA) was more frequent in MS patients (28%) than controls (10%) (OR= 3.52, 95%CI=1.16-10.72, p=0.027). Regarding the rs2213585, rs2213586 there were no significance associations between control group and patients.
Conclusion(s):
This is the first study that revealed that HLA-DRB1 15.01genotyp may be considered as genetic risk factor for MS susceptibility in Iraqi MS patients until 2020